RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PUS10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106874-100
Catalog Number: LS-G106874
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PUS10 (NM_144709), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC139 | DOBI | Pseudouridine synthase 10 | Pseudouridylate synthase 10 | TRNA pseudouridylate synthase | TRNA-uridine isomerase | TRNA pseudouridine 55 synthase | PUS10 | Psi55 synthase
- Origin Species
- Human
- NCBI GenBank
- AA213749
- SwissProt
- Q3MIT2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFPLTEENKH VAQLLLNTGT CPRCIFRFCG VDFHAPYKLP YKELLNELQK FLETEKDELI61LEVMNPPPKK IRLQELEDSI DNLSQNGEGR ISVSHVGSTA SKNSNLNVCN VCLGILQEFC121EKDFIKKVCQ KVEASGFEFT SLVFSVSFPP QLSVREHAAW LLVKQEMGKQ SLSLGRDDIV181QLKEAYKWIT HPLFSEELGV PIDGKSLFEV SVVFAHPETV EDCHFLAAIC PDCFKPAKNK241QSVFTRMAVM KALNKIKEED FLKQFPCPPN SPKAVCAVLE IECAHGAVFV AGRYNKYSRN301LPQTPWIIDG ERKLESSVEE LISDHLLAVF KAESFNFSSS GREDVDVRTL GNGRPFAIEL361VNPHRVHFTS QEIKELQQKI NNSSNKIQVR DLQLVTREAI GHMKEGEEEK TKTYSALIWT421NKAIQKKDIE FLNDIKDLKI DQKTPLRVLH RRPLAVRARV IHFMETQYVD EHHFRLHLKT481QAGTYIKEFV HGDFGRTKPN IGSLMNVTAD ILELDVESVD VDWPPALDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
529 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
