RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PUS1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122793-100
Catalog Number: LS-G122793
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PUS1 (NM_001002019), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MLASA1 | PUS1 | TRNA uridine isomerase I | Pseudouridylate synthase 1 | TRNA-uridine isomerase I
- Origin Species
- Human
- NCBI GenBank
- NM_025215
- SwissProt
- Q9Y606
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGNAEPPPA GAACPQDRRS CSGRAGGDRV WEDGEHPAKK LKSGGDEERR EKPPKRKIVL61LMAYSGKGYH GMQRNVGSSQ FKTIEDDLVS ALVRSGCIPE NHGEDMRKMS FQRCARTDKG121VSAAGQVVSL KVWLIDDILE KINSHLPSHI RILGLKRVTG GFNSKNRCDA RTYCYLLPTF181AFAHKDRDVQ DETYRLSAET LQQVNRLLAC YKGTHNFHNF TSQKGPQDPS ACRYILEMYC241EEPFVREGLE FAVIRVKGQS FMMHQIRKMV GLVVAIVKGY APESVLERSW GTEKVDVPKA301PGLGLVLERV HFEKYNQRFG NDGLHEPLDW AQEEGKVAAF KEEHIYPTII GTERDERSMA361QWLSTLPIHN FSATALTAGG TGAKVPSPLE GSEGDGDTDT RTRPLEQKLI SEEDLAANDI421LDYKDDDDKV
430 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
