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PPP2R1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP2R1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G130070-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R1B (NM_181700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PPP2R1B | PR65B | PP2A subunit A isoform R1-beta | PP2A-Abeta
Origin Species
Human
NCBI GenBank
NM_002716
SwissProt
P30154

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGASELGTG PGAAGGDGDD SLYPIAVLID ELRNEDVQPP LENLATVEET VVRDKAVESL
61RQISQEHTPV ALEAYFVPLV KRLASGDWFT SRTSACGLFS VCYPRASNAV KAEIRQQFRS
121LCSDDTPMVR RAAASKLGEF AKVLELDSVK SEIVPLFTSL ASDEQDSVRL LAVEACVSIA
181QLLSQDDLET LVMPTLRQAA EDKSWRVRYM VADRFSELQK AMGPKITLND LIPAFQNLLK
241DCEAEVRAAA AHKVKELGEN LPIEDRETII MNQILPYIKE LVSDTNQHVK SALASVIMGL
301STILGKENTI EHLLPLFLAQ LKDECPDVRL NIISNLDCVN EVIGIRQLSQ SLLPAIVELA
361EDAKWRVRLA IIEYMPLLAG QLGVEFFDEK LNSLCMAWLV DHVYAIREAA TNNLMKLVQK
421FGTEWAQNTI VPKVLVMAND PNYLHRMTTL FCINALSEAC GQEITTKQML PIVLKMAGDQ
481VANVRFNVAK SLQKIGPILD TNALQGEVKP VLQKLGQDED MDVKYFAQEA ISVVAQRLRK
541LEFPVKDSGE PSVPRADKNH FPRPTVPGED MGKGPVYQLR GDTRDTLAQL GIAELVHFSQ
601STD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.