Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLS3 / T Plastin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130067-100
Catalog Number: LS-G130067
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human T Plastin(PLS3) (NM_001172335), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- T plastin | T-plastin | T fimbrin | Plastin 3 | Plastin-3 | PLS3 | Plastin 3 (T isoform)
- Origin Species
- Hu
- NCBI GenBank
- NM_005032
- SwissProt
- P13797
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 68 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDEMATTQIS KDELDELKEA FAKVDLNSNG FICDYELHEL FKEANMPLPG YKIFQEVKSS61DIAKTFRKAI NRKEGICALG GTSELSSEGT QHSYSEEEKY AFVNWINKAL ENDPDCRHVI121PMNPNTDDLF KAVGDGIVLC KMINLSVPDT IDERAINKKK LTPFIIQENL NLALNSASAI181GCHVVNIGAE DLRAGKPHLV LGLLWQIIKI GLFADIELSR NEALAALLRD GETLEELMKL241SPEELLLRWA NFHLENSGWQ KINNFSADIK DSKAYFHLLN QIAPKGQKEG EPRIDINMSG301FNETDDLKRA ESMLQQADKL GCRQFVTPAD VVSGNPKLNL AFVANLFNKY PALTKPENQD361IDWTLLEGET REERTFRNWM NSLGVNPHVN HLYADLQDAL VILQLYERIK VPVDWSKVNK421PPYPKLGANM KKLENCNYAV ELGKHPAKFS LVGIGGQDLN DGNQTLTLAL VWQLMRRYTL481NVLEDLGDGQ KANDDIIVNW VNRTLSEAGK STSIQSFKDK TISSSLAVVD LIDAIQPGCI541NYDLVKSGNL TEDDKHNNAK YAVSMARRIG ARVYALPEDL VEVKPKMVMT VFACLMGRGM601KRVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
603 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.