Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PIBF1 / PIBF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100794-100
Catalog Number: LS-G100794
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C13orf24(PIBF1) (NM_006346), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C13orf24 | CEP90 | PIBF1 | RP11-505F3.1 | PIBF
- Origin Species
- Human
- NCBI GenBank
- NM_006346
- SwissProt
- Q8WXW3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 89.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRKISKESK KVNISSSLES EDISLETTVP TDDISSSEER EGKVRITRQL IERKELLHNI61QLLKIELSQK TMMIDNLKVD YLTKIEELEE KLNDALHQKQ LLTLRLDNQL AFQQKDASKY121QELMKQEMET ILLRQKQLEE TNLQLREKAG DVRRNLRDFE LTEEQYIKLK AFPEDQLSIP181EYVSVRFYEL VNPLRKEICE LQVKKNILAE ELSTNKNQLK QLTETYEEDR KNYSEVQIRC241QRLALELADT KQLIQQGDYR QENYDKVKSE RDALEQEVIE LRRKHEILEA SHMIQTKERS301ELSKEVVTLE QTVTLLQKDK EYLNRQNMEL SVRCAHEEDR LERLQAQLEE SKKAREEMYE361KYVASRDHYK TEYENKLHDE LEQIRLKTNQ EIDQLRNASR EMYERENRNL REARDNAVAE421KERAVMAEKD ALEKHDQLLD RYRELQLSTE SKVTEFLHQS KLKSFESERV QLLQEETARN481LTQCQLECEK YQKKLEVLTK EFYSLQASSE KRITELQAQN SEHQARLDIY EKLEKELDEI541IMQTAEIENE DEAERVLFSY GYGANVPTTA KRRLKQSVHL ARRVLQLEKQ NSLILKDLEH601RKDQVTQLSQ ELDRANSLLN QTQQPYRYLI ESVRQRDSKI DSLTESIAQL EKDVSNLNKE661KSALLQTKNQ MALDLEQLLN HREELAAMKQ ILVKMHSKHS ENSLLLTKTE PKHVTENQKS721KTLNVPKEHE DNIFTPKPTL FTKKEAPEWS KKQKMKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
757 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.