Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PGM1 / Phosphoglucomutase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130043-100
Catalog Number: LS-G130043
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PGM1 (NM_001172818), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDG1T | Glucose phosphomutase 1 | GSD14 | PGM1 | Phosphoglucomutase 1 | PGM 1 | Phosphoglucomutase-1 | Phosphoglucomutase
- Origin Species
- Hu
- NCBI GenBank
- NM_002633
- SwissProt
- P36871
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 64.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDFEEWISG TYRKMEEGPL PLLTFATAPY HDQKPGTSGL RKKTYYFEEK PCYLENFIQS61IFFSIDLKDR QGSSLVVGGD GRYFNKSAIE TIVQMAAANG IGRLVIGQNG ILSTPAVSCI121IRKIKAIGGI ILTASHNPGG PNGDFGIKFN ISNGGPAPEA ITDKIFQISK TIEEYAVCPD181LKVDLGVLGK QQFDLENKFK PFTVEIVDSV EAYATMLRSI FDFSALKELL SGPNRLKIRI241DAMHGVVGPY VKKILCEELG APANSAVNCV PLEDFGGHHP DPNLTYAADL VETMKSGEHD301FGAAFDGDGD RNMILGKHGF FVNPSDSVAV IAANIFSIPY FQQTGVRGFA RSMPTSGALD361RVASATKIAL YETPTGWKFF GNLMDASKLS LCGEESFGTG SDHIREKDGL WAVLAWLSIL421ATRKQSVEDI LKDHWQKYGR NFFTRYDYEE VEAEGANKMM KDLEALMFDR SFVGKQFSAN481DKVYTVEKAD NFEYSDPVDG SISRNQGLRL IFTDGSRIVF RLSGTGSAGA TIRLYIDSYE541KDVAKINQDP QVMLAPLISI ALKVSQLQER TGRTAPTVITTRTRPLEQKLISEEDLAANDILDYKDDDDKV
580 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.