Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PEX2 / PAF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G123269-100
Catalog Number: LS-G123269
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PEX2 (NM_000318), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PBD5B | PBD5A | Peroxisome assembly factor 1 | Peroxisome assembly factor-1 | PMP35 | PXMP3 | RNF72 | PAF-1 | ZWS3 | Peroxin-2 | Peroxisomal membrane protein 3 | Peroxisome biogenesis factor 2 | PMP3 | PEX2 | RING finger protein 72
- Origin Species
- Hu
- NCBI GenBank
- NM_000318
- SwissProt
- P28328
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 34.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASRKENAKS ANRVLRISQL DALELNKALE QLVWSQFTQC FHGFKPGLLA RFEPEVKACL61WVFLWRFTIY SKNATVGQSV LNIKYKNDFS PNLRYQPPSK NQKIWYAVCT IGGRWLEERC121YDLFRNHHLA SFGKVKQCVN FVIGLLKLGG LINFLIFLQR GKFATLTERL LGIHSVFCKP181QNIREVGFEY MNRELLWHGF AEFLIFLLPL INVQKLKAKL SSWCIPLTGA PNSDNTLATS241GKECALCGEW PTMPHTIGCE HIFCYFCAKS SFLFDVYFTC PKCGTEVHSL QPLKSGIEMS301EVNALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
305 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.