RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PAPLN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105346-100
Catalog Number: LS-G105346
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAPLN (NM_173462), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Papilin | PAPLN
- Origin Species
- Human
- SwissProt
- O95428
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLLLLVPLL LAPAPGSSAP KVRRQSDTWG PWSQWSPCSR TCGGGVSFRE RPCYSQRRDG61GSSCVGPARS HRSCRTESCP DGARDFRAEQ CAEFDGAEFQ GRRYRWLPYY SAPNKCELNC121IPKGENFYYK HREAVVDGTP CEPGKRDVCV DGSCRVVGCD HELDSSKQED KCLRCGGDGT181TCYPVAGTFD ANDLSRAVKN VRGEYYLNGH WTIEAARALP AASTILHYER GAEGDLAPER241LHARGPTSEP LVIELISQEP NPGVHYEYHL PLRRPSPGFS WSHGSWSDCS AECGGGHQSR301LVFCTIDHEA YPDHMCQRQP RPADRRSCNL HPCPETKRWK AGPWAPCSAS CGGGSQSRSV361YCISSDGAGI QEAVEEAECA GLPGKPPAIQ ACNLQRCAAW SPEPWGECSV SCGVGVRKRS421VTCRGERGSL LHTAACSLED RPPLTEPCVH EDCPLLSDQA WHVGTWGLCS KSCSSGTRRR481QVICAIGPPS HCGSLQHSKP VDVEPCNTQP CHLPQEVPSM QDVHTPASNP WMPLGPQESP541ASDSRGQWWA AQEHPSARGD HRGERGDPRG DQGTHLSALG PAPSLQQPPY QQPLRSGSGP601HDCRHSPHGC CPDGHTASLG PQWQGCPGAP CQQSRYGCCP DRVSVAEGPH HAGCTKSYGG661DSTGGMPRSR AVASTVHNTH QPQAQQNEPS ECRGSQFGCC YDNVATAAGP LGEGCVGQPS721HAYPVRCLLP SAHGSCADWA ARWYFVASVG QCNRFWYGGC HGNANNFASE QECMSSCQGS781LHGPRRPQPG ASGRSTHTDG GGSSPAGEQE PSQHRTGAAV QRKPWPSGGL WRQDQQPGPG841EAPHTQAFGE WPWGQELGSR APGLGGDAGS PAPPFHSSSY RISLAGVEPS LVQAALGQLV901RLSCSDDTAP ESQAAWQKDG QPISSDRHRL QFDGSLIIHP LQAEDAGTYS CGSTRPGRDS961QKIQLRIIGG DMAVLSEAEL SRFPQPRDPA QDFGQAGAAG PLGAIPSSHP QPANRLRLDQ1021NQPRVVDASP GQRIRMTCRA EGFPPPAIEW QRDGQPVSSP RHQLQPDGSL VISRVAVEDG1081GFYTCVAFNG QDRDQRWVQL RVLGELTISG LPPTVTVPEG DTARLLCVVA GESVNIRWSR1141NGLPVQADGH RVHQSPDGTL LIYNLRARDE GSYTCSAYQG SQAVSRSTEV KVVSPAPTAQ1201PRDPGRDCVD QPELANCDLI LQAQLCGNEY YSSFCCASCS RFQPHAQPIW QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1251 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
