LSBio
PABPC1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PABPC1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PABPC1L antibodies →
$504.00each
Catalog Number: LS-G124489-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PABPC1L (NM_001124756), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf119 | EPAB | PABPC1L | PABPC1L1 | DJ1069P2.3
Origin Species
Human
NCBI GenBank
AK026760

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNASGSGYPL ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RVCRDVATRR SLGYAYINFQ
61QPADAERALD TMNFEMLKGQ PIRIMWSQRD PGLRKSGVGN IFIKNLEDSI DNKALYDTFS
121TFGNILSCKV ACDEHGSRGF GFVHFETHEA AQQAINTMNG MLLNDRKVFV GHFKSRRERE
181AELGARALEF TNIYVKNLPV DVDEQGLQDL FSQFGKMLSV KVMRDNSGHS RCFGFVNFEK
241HEEAQKAVVH MNGKEVSGRL LYAGRAQKRV ERQNELKRRF EQMKQDRLRR YQGVNLYVKN
301LDDSIDDDKL RKEFSPYGVI TSAKVMTEGG HSKGFGFVCF SSPEEATKAV TEMNGRIVGT
361KPLYVALAQR KEERKAILTN QYMQRLSTMR TLSNPLLGSF QQPSSYFLPA MPQPPAQAAY
421YGCGPVTPTQ PAPRWTSQPP RPSCASMVRP PVVPRRPPAH ISSVRQASTQ VPRTVPHTQR
481VANIGTQTTG PSGVGCCTPG RPLLPCKCSS AAHSTYRVQE PAVHIPGQEP LTASMLAAAP
541LHEQKQMIGE RLYPLIHDVH TQLAGKITGM LLEIDNSELL LMLESPESLH AKIDEAVAVL
601QAHQAMEQPK AYMH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

614 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.