Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ONECUT1 / HNF6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100312-100
Catalog Number: LS-G100312
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HNF6(ONECUT1) (NM_004498), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hepatocyte nuclear factor 6 | HNF6 | One cut domain family member 1 | One cut homeobox 1 | HNF-6 | HNF6A | ONECUT1
- Origin Species
- Human
- NCBI GenBank
- U77975
- SwissProt
- Q9UBC0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVAH RGSHLPPAHP RSMGMASLLD61GGSGGGDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP TTYTTLTPLQ PLPPISTVSD121KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT LMRDERGLAS MNNLYTPYHK DVAGMGQSLS181PLSSSGLGSI HNSQQGLPHY AHPGAAMPTD KMLTPNGFEA HHPAMLGRHG EQHLTPTSAG241MVPINGLPPH HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR301ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM WKWLQEPEFQ361RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL HAIFKENKRP SKELQITISQ421QLGLELSTVS NFFMNARRRS LDKWQDEGSS NSGNSSSSSS TCTKATRTRPLEQKLISEEDLAANDILDYKDDDDKV
465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.