Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NRXN1 / Neurexin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G125496-100
Catalog Number: LS-G125496
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neurexin 1(NRXN1) (NM_001135659), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hs.22998 | KIAA0578 | Neurexin I-alpha | Neurexin-1-alpha | NRXN1 | PTHSL2 | Neurexin 1 | Neurexin I-beta | Neurexin I | Neurexin-1-beta | SCZD17
- Origin Species
- Human
- NCBI GenBank
- NM_004801
- SwissProt
- Q9ULB1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 169.91 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTALLQRGG CFLLCLSLLL LGCWAELGSG LEFPGAEGQW TRFPKWNACC ESEMSFQLKT61RSARGLVLYF DDEGFCDFLE LILTRGGRLQ LSFSIFCAEP ATLLADTPVN DGAWHSVRIR121RQFRNTTLFI DQVEAKWVEV KSKRRDMTVF SGLFVGGLPP ELRAAALKLT LASVREREPF181KGWIRDVRVN SSQVLPVDSG EVKLDDEPPN SGGGSPCEAG EEGEGGVCLN GGVCSVVDDQ241AVCDCSRTGF RGKDCSQEIK FGLQCVLPVL LHDNDQGKYC CINTAKPLTE KDNNVEGLAH301LMMGDQGKSK GKEEYIATFK GSEYFCYDLS QNPIQSSSDE ITLSFKTLQR NGLMLHTGKS361ADYVNLALKN GAVSLVINLG SGAFEALVEP VNGKFNDNAW HDVKVTRNLR QHSGIGHAMV421NKLHCSVTIS VDGILTTTGY TQEDYTMLGS DDFFYVGGSP STADLPGSPV SNNFMGCLKE481VVYKNNDVRL ELSRLAKQGD PKMKIHGVVA FKCENVATLD PITFETPESF ISLPKWNAKK541TGSISFDFRT TEPNGLILFS HGKPRHQKDA KHPQMIKVDF FAIEMLDGHL YLLLDMGSGT601IKIKALLKKV NDGEWYHVDF QRDGRSGTIS VNTLRTPYTA PGESEILDLD DELYLGGLPE661NKAGLVFPTE VWTALLNYGY VGCIRDLFID GQSKDIRQMA EVQSTAGVKP SCSKETAKPC721LSNPCKNNGM CRDGWNRYVC DCSGTGYLGR SCEREATVLS YDGSMFMKIQ LPVVMHTEAE781DVSLRFRSQR AYGILMATTS RDSADTLRLE LDAGRVKLTV NLDCIRINCN SSKGPETLFA841GYNLNDNEWH TVRVVRRGKS LKLTVDDQQA MTGQMAGDHT RLEFHNIETG IITERRYLSS901VPSNFIGHLQ SLTFNGMAYI DLCKNGDIDY CELNARFGFR NIIADPVTFK TKSSYVALAT961LQAYTSMHLF FQFKTTSLDG LILYNSGDGN DFIVVELVKG YLHYVFDLGN GANLIKGSSN1021KPLNDNQWHN VMISRDTSNL HTVKIDTKIT TQITAGARNL DLKSDLYIGG VAKETYKSLP1081KLVHAKEGFQ GCLASVDLNG RLPDLISDAL FCNGQIERGC EGPSTTCQED SCSNQGVCLQ1141QWDGFSCDCS MTSFSGPLCN DPGTTYIFSK GGGQITYKWP PNDRPSTRAD RLAIGFSTVQ1201KEAVLVRVDS SSGLGDYLEL HIHQGKIGVK FNVGTDDIAI EESNAIINDG KYHVVRFTRS1261GGNATLQVDS WPVIERYPAG NNDNERLAIA RQRIPYRLGR VVDEWLLDKG RQLTIFNSQA1321TIIIGGKEQG QPFQGQLSGL YYNGLKVLNM AAENDANIAI VGNVRLVGEV PSSMTTESTA1381TAMQSEMSTS IMETTTTLAT STARRGKPPT KEPISQTTDD ILVASAECPS DDEDIDPCEP1441SSGGLANPTR AGGREPYPGS AEVIRESSST TGMVVGIVAA AALCILILLY AMYKYRNRDE1501GSYHVDESRN YISNSAQSNG AVVKEKQPSS AKSSNKNKKN KDKEYYVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1547 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.