Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NFATC4 / NFAT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G125612-100
Catalog Number: LS-G125612
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFATC4 (NM_001136022), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NF-ATc4 | NFAT3 | NF-AT3 | NFATC4
- Origin Species
- Human
- NCBI GenBank
- NM_004554
- SwissProt
- Q14934
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MITTLPSLLP ASLASISHRV TNLPSNSLSH NPGLSKPDFP GNSSPGLPSS SSPGRDLGAP61AGSMGAASCE DEELEFKLVF GEEKEAPPLG AGGLGEELDS EDAPPCCRLA LGEPPPYGAA121PIGIPRPPPP RPGMHSPPPR PAPSPGTWES QPARSVRLGG PGGGAGGAGG GRVLECPSIR181ITSISPTPEP PAALEDNPDA WGDGSPRDYP PPEGFGGYRE AGGQGGGAFF SPSPGSSSLS241SWSFFSDASD EAALYAACDE VESELNEAAS RFGLGSPLPS PRASPRPWTP EDPWSLYGPS301PGGRGPEDSW LLLSAPGPTP ASPRPASPCG KRRYSSSGTP SSASPALSRR GSLGEEGSEP361PPPPPLPLAR DPGSPGPFDY VGAPPAESIP QKTRRTSSEQ AVALPRSEEP ASCNGKLPLG421AEESVAPPGG SRKEVAGMDY LAVPSPLAWS KARIGGHSPI FRTSALPPLD WPLPSQYEQL481ELRIEVQPRA HHRAHYETEG SRGAVKAAPG GHPVVKLLGY SEKPLTLQMF IGTADERNLR541PHAFYQVHRI TGKMVATASY EAVVSGTKVL EMTLLPENNM AANIDCAGIL KLRNSDIELR601KGETDIGRKN TRVRLVFRVH VPQGGGKVVS VQAASVPIEC SQRSAQELPQ VEAYSPSACS661VRGGEELVLT GSNFLPDSKV VFIERGPDGK LQWEEEATVN RLQSNEVTLT LTVPEYSNKR721VSRPVQVYFY VSNGRRKRSP TQSFRFLPVI CKEEPLPDSS LRGFPSASAT PFGTDMDFSP781PRPPYPSYPH EDPACETPYL SEGFGYGMPP LYPQTGPPPS YRPGLRMFPE TRGTTGCAQP841PAVSFLPRPF PSDPYGGRGS SFSLGLPFSP PAPFRPPPLP ASPPLEGPFP SQSDVHPLPA901EGYNKVGPGY GPGEGAPEQE KSRGGYSSGF RDSVPIQGIT LEEGGCGTGG CECECVQEIA961LHVCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
964 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.