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MTF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MTF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MTF1 antibodies →
$504.00each
Catalog Number: LS-G100688-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTF1 (NM_005955), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MTF-1 | Transcription factor MTF-1 | MTF1 | Zinc regulatory factor | ZRF
Origin Species
Human
NCBI GenBank
NM_005955
SwissProt
Q14872

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGEHSPDNNI IYFEAEEDEL TPDDKMLRFV DKNGLVPSSS GTVYDRTTVL IEQDPGTLED
61EDDDGQCGEH LPFLVGGEEG FHLIDHEAMS QGYVQHIISP DQIHLTINPG STPMPRNIEG
121ATLTLQSECP ETKRKEVKRY QCTFEGCPRT YSTAGNLRTH QKTHRGEYTF VCNQEGCGKA
181FLTSYSLRIH VRVHTKEKPF ECDVQGCEKA FNTLYRLKAH QRLHTGKTFN CESEGCSKYF
241TTLSDLRKHI RTHTGEKPFR CDHDGCGKAF AASHHLKTHV RTHTGERPFF CPSNGCEKTF
301STQYSLKSHM KGHDNKGHSY NALPQHNGSE DTNHSLCLSD LSLLSTDSEL RENSSTTQGQ
361DLSTISPAII FESMFQNSDD TAIQEDPQQT ASLTESFNGD AESVSDVPPS TGNSASLSLP
421LVLQPGLSEP PQPLLPASAP SAPPPAPSLG PGSQQAAFGN PPALLQPPEV PVPHSTQFAA
481NHQEFLPHPQ APQPIVPGLS VVAGASASAA AVASAVAAPA PPQSTTEPLP AMVQTLPLGA
541NSVLTNNPTI TITPTPNTAI LQSSLVMGEQ NLQWILNGAT SSPQNQEQIQ QASKVEKVFF
601TTAVPVASSP GSSVQQIGLS VPVIIIKQEE ACQCQCACRD SAKERASSRR KGCSSPPPPE
661PSPQAPDGPS LQLPAQTFSS APVPGSSSST LPSSCEQSRQ AETPSDPQTE TLSAMDVSEF
721LSLQSLDTPS NLIPIEALLQ GEEEMGLTSS FSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

753 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.