Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MSLN / Mesothelin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100652-100
Catalog Number: LS-G100652
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Mesothelin(MSLN) (NM_005823), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAK1 antigen | MPF | SMRP | Mesothelin | MSLN | CAK1
- Origin Species
- Human
- NCBI GenBank
- NM_013404
- SwissProt
- Q13421
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.91 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALPTARPLL GSCGTPALGS LLFLLFSLGW VQPSRTLAGE TGQAAPLDGV LANPPNISSL61SPRQLLGFPC AEVSGLSTER VRELAVALAQ KNVKLSTEQL RCLAHRLSEP PEDLDALPLD121LLLFLNPDAF SGPQACTRFF SRITKANVDL LPRGAPERQR LLPAALACWG VRGSLLSEAD181VRALGGLACD LPGRFVAESA EVLLPRLVSC PGPLDQDQQE AARAALQGGG PPYGPPSTWS241VSTMDALRGL LPVLGQPIIR SIPQGIVAAW RQRSSRDPSW RQPERTILRP RFRREVEKTA301CPSGKKAREI DESLIFYKKW ELEACVDAAL LATQMDRVNA IPFTYEQLDV LKHKLDELYP361QGYPESVIQH LGYLFLKMSP EDIRKWNVTS LETLKALLEV NKGHEMSPQV ATLIDRFVKG421RGQLDKDTLD TLTAFYPGYL CSLSPEELSS VPPSSIWAVR PQDLDTCDPR QLDVLYPKAR481LAFQNMNGSE YFVKIQSFLG GAPTEDLKAL SQQNVSMDLA TFMKLRTDAV LPLTVAEVQK541LLGPHVEGLK AEERHRPVRD WILRQRQDDL DTLGLGLQGG IPNGYLVLDL SVQEALSGTP601CLLGPGPVLT VLALLLASTL ATRTRPLEQK LISEEDLAAN DILDYKDDDD KV
652 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.