LSBio
Mouse CD40L Protein (Recombinant Human IgG1 Fc, N-terminus)

Measured by its binding ability in a functional ELISA. Immobilized mouse CD40L-Fc at 10 µg/mL (100 µl/well) can bind biotinylated mouse CD40-Fch, The EC50 of biotinylated mouse CD40-Fch is 33-49 ng/mL.

Mouse CD40L Protein (Recombinant Human IgG1 Fc, N-terminus)

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Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
See all CD40LG antibodies →
$372.00each
Catalog Number: LS-G49405-50

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Overview

Description
CD40L Protein LS-G49405 is a Recombinant Mouse CD40L produced in HEK 293 Cells Gly115-Leu260 with Human IgG1 Fc, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD40LG | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Origin Species
Mouse
NCBI GenBank
NM_000074
SwissProt
P29965

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
Human IgG1 Fc Tag
Molecular Weight
The recombinant mouse CD40LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 406 amino acids and has a calculated molecular mass of 44.4 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
Protein Type
Recombinant
Amino Acid Sequence
1Gly115-Leu 260

13 aa

Format

Purity
>80%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

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