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LYN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LYN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LYN antibodies →
$504.00each
Catalog Number: LS-G124227-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LYN (NM_001111097), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Lyn protein tyrosine kinase | Lyn ptk | Lyn tyrosine kinase | p56Lyn | p53Lyn | Tyrosine-protein kinase Lyn | JTK8 | LYN | Lyn kinase
Origin Species
Human
NCBI GenBank
NM_002350
SwissProt
P07948

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCIKSKGKD SLSDDGVDLK TQPVPESQLL PGQRFQTKDP EEQGDIVVAL YPYDGIHPDD
61LSFKKGEKMK VLEEHGEWWK AKSLLTKKEG FIPSNYVAKL NTLETEEWFF KDITRKDAER
121QLLAPGNSAG AFLIRESETL KGSFSLSVRD FDPVHGDVIK HYKIRSLDNG GYYISPRITF
181PCISDMIKHY QKQADGLCRR LEKACISPKP QKPWDKDAWE IPRESIKLVK RLGAGQFGEV
241WMGYYNNSTK VAVKTLKPGT MSVQAFLEEA NLMKTLQHDK LVRLYAVVTR EEPIYIITEY
301MAKGSLLDFL KSDEGGKVLL PKLIDFSAQI AEGMAYIERK NYIHRDLRAA NVLVSESLMC
361KIADFGLARV IEDNEYTARE GAKFPIKWTA PEAINFGCFT IKSDVWSFGI LLYEIVTYGK
421IPYPGRTNAD VMTALSQGYR MPRVENCPDE LYDIMKMCWK EKAEERPTFD YLQSVLDDFY
481TATEGQYQQQ P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.