RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LSG1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111761-100
Catalog Number: LS-G111761
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LSG1 (NM_018385), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HLsg1 | Large subunit GTPase 1 homolog | LSG1
- Origin Species
- Human
- NCBI GenBank
- NM_018385
- SwissProt
- Q9H089
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRRRAPAGG SLGRALMRHQ TQRSRSHRHT DSWLHTSELN DGYDWGRLNL QSVTEQSSLD61DFLATAELAG TEFVAEKLNI KFVPAEARTG LLSFEESQRI KKLHEENKQF LCIPRRPNWN121QNTTPEELKQ AEKDNFLEWR RQLVRLEEEQ KLILTPFERN LDFWRQLWRV IERSDIVVQI181VDARNPLLFR CEDLECYVKE MDANKENVIL INKADLLTAE QRSAWAMYFE KEDVKVIFWS241ALAGAIPLNG DSEEEANRDD RQSNTTEFGH SSFDQAEISH SESEHLPARD SPSLSENPTT301DEDDSEYEDC PEEEEDDWQT CSEEDGPKEE DCSQDWKESS TADSEARSRK TPQKRQIHNF361SHLVSKQELL ELFKELHTGR KVKDGQLTVG LVGYPNVGKS STINTIMGNK KVSVSATPGH421TKHFQTLYVE PGLCLCDCPG LVMPSFVSTK AEMTCSGILP IDQMRDHVPP VSLVCQNIPR481HVLEATYGIN IITPREDEDP HRPPTSEELL TAYGYMRGFM TAHGQPDQPR SARYILKDYV541SGKLLYCHPP PGRDPVTFQH QHQRLLENKM NSDEIKMQLG RNKKAKQIEN IVDKTFFHQE601NVRALTKGVQ AVMGYKPGSG VVTASTASSE NGAGKPWKKH GNRNKKEKSR RLYKHLDMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
658 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
