Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LILRB5 / LIR8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119743-100
Catalog Number: LS-G119743
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LILRB5 (NM_001081442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD85c antigen | LILRB5 | LIR-8 | LIR8 | CD85C
- Origin Species
- Human
- NCBI GenBank
- NM_006840
- SwissProt
- O75023
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLTLSVLIC LGLSVGPRTC VQAGTLPKPT LWAEPASVIA RGKPVTLWCQ GPLETEEYRL61DKEGLPWARK RQNPLEPGAK AKFRIPSTVY DSAGRYRCYY ETPAGWSEPS DPLELVATGF121YAEPTLLALP SPVVASGGNV TLQCDTLDGL LTFVLVEEEQ KLPRTLYSQK LPKGPSQALF181PVGPVTPSCR WRFRCYYYYR KNPQVWSNPS DLLEILVPGV SRKPSLLIPQ GSVVARGGSL241TLQCRSDVGY DIFVLYKEGE HDLVQGSGQQ PQAGLSQANF TLGPVSRSHG GQYRCYGAHN301LSPRWSAPSD PLDILIAGLI PDIPALSVQP GPKVASGENV TLLCQSWHQI DTFFLTKEGA361AHPPLCLKSK YQSYRHQAEF SMSPVTSAQG GTYRCYSAIR SYPYLLSSPS YPQELVVSGP421SGDPSLSPTG STPTPGPEDQ PLTPTGLDPQ SGLGRHLGVV TGVSVAFVLL LFLLLFLLLR481HRHQSKHRTS AHFYRPAGAA GPEPKDQGLQ KRASPVADIQ EEILNAAVKD TQPKDGVEMD541ARAAASEAPQ DVTYAQLHSL TLRREATEPP PSQEREPPAE PSIYAPLAIHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
590 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.