Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LDLR / LDL Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G131096-100
Catalog Number: LS-G131096
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LDL Receptor(LDLR) (NM_001195800), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FHC | LDL receptor | LDLCQ2 | LDLR
- Origin Species
- Hu
- NCBI GenBank
- NM_000527
- SwissProt
- P01130
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 77.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPWGWKLRW TVALLLAAAG TAVGDRCERN EFQCQDGKCI SYKWVCDGSA ECQDGSDESQ61ETCLSVTCKS GDFSCGGRVN RCIPQFWRCD GQVDCDNGSD EQGCLTLCEG PNKFKCHSGE121CITLDKVCNM ARDCRDWSDE PIKECGTNEC LDNNGGCSHV CNDLKIGYEC LCPDGFQLVA181QRRCEDIDEC QDPDTCSQLC VNLEGGYKCQ CEEGFQLDPH TKACKAVGSI AYLFFTNRHE241VRKMTLDRSE YTSLIPNLRN VVALDTEVAS NRIYWSDLSQ RMICSTQLDR AHGVSSYDTV301ISRDIQAPDG LAVDWIHSNI YWTDSVLGTV SVADTKGVKR KTLFRENGSK PRAIVVDPVH361GFMYWTDWGT PAKIKKGGLN GVDIYSLVTE NIQWPNGITL DLLSGRLYWV DSKLHSISSI421DVNGGNRKTI LEDEKRLAHP FSLAVFEDKV FWTDIINEAI FSANRLTGSD VNLLAENLLS481PEDMVLFHNL TQPRGVNWCE RTTLSNGGCQ YLCLPAPQIN PHSPKFTCAC PDGMLLARDM541RSCLTEAEAA VATQETSTVR LKVSSTAVRT QHTTTRPVPD TSRLPGATPG LTTVEIVTMS601HQALGDVAGR GNEKKPSSVR ALSIVLPIVL LVFLCLGVFL LWKNWRLKNI NSINFDNPVY661QKTTEDEVHI CHNQDGYSYP SRQMVSLEDD VATRTRPLEQKLISEEDLAANDILDYKDDDDKV
692 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.