Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LDLR / LDL Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G131090-100
Catalog Number: LS-G131090
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LDL Receptor(LDLR) (NM_001195803), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FHC | LDL receptor | LDLCQ2 | LDLR
- Origin Species
- Hu
- NCBI GenBank
- NM_000527
- SwissProt
- P01130
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 76.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPWGWKLRW TVALLLAAAG TAVGDRCERN EFQCQDGKCI SYKWVCDGSA ECQDGSDESQ61ETCLSVTCKS GDFSCGGRVN RCIPQFWRCD GQVDCDNGSD EQGCPVATCR PDEFQCSDGN121CIHGSRQCDR EYDCKDMSDE VGCVNVTLCE GPNKFKCHSG ECITLDKVCN MARDCRDWSD181EPIKECGTNE CLDNNGGCSH VCNDLKIGYE CLCPDGFQLV AQRRCEDIDE CQDPDTCSQL241CVNLEGGYKC QCEEGFQLDP HTKACKAVGS IAYLFFTNRH EVRKMTLDRS EYTSLIPNLR301NVVALDTEVA SNRIYWSDLS QRMICSTQLD RAHGVSSYDT VISRDIQAPD GLAVDWIHSN361IYWTDSVLGT VSVADTKGVK RKTLFRENGS KPRAIVVDPV HGFMYWTDWG TPAKIKKGGL421NGVDIYSLVT ENIQWPNGIT LDLLSGRLYW VDSKLHSISS IDVNGGNRKT ILEDEKRLAH481PFSLAVFEDK VFWTDIINEA IFSANRLTGS DVNLLAENLL SPEDMVLFHN LTQPREAEAA541VATQETSTVR LKVSSTAVRT QHTTTRPVPD TSRLPGATPG LTTVEIVTMS HQALGDVAGR601GNEKKPSSVR ALSIVLPIVL LVFLCLGVFL LWKNWRLKNI NSINFDNPVY QKTTEDEVHI661CHNQDGYSYP SRQMVSLEDD VATRTRPLEQKLISEEDLAANDILDYKDDDDKV
682 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.