RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
JRK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120031-100
Catalog Number: LS-G120031
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JRK (NM_001077527), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Jerky (mouse) homolog | Jerky protein homolog | JH8 | Jerky homolog (mouse) | JRK
- Origin Species
- Human
- NCBI GenBank
- NM_003724
- SwissProt
- O75564
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASKPAAGKS RGEKRKRVVL TLKEKIDICT RLEKGESRKA LMQEYNVGMS TLYDIRAHKA61QLLRFFASSD SNKALEQRRT LHTPKLEHLD RVLYEWFLGK RSEGVPVSGP MLIEKAKDFY121EQMQLTEPCV FSGGWLWRFK ARHGIKKLDA SSEKQSADHQ AAEQFCAFFR SLAAEHGLSA181EQVYNADETG LFWRCLPNPT PEGGAVPGPK QGKDRLTVLM CANATGSHRL KPLAIGKCSG241PRAFKGIQHL PVAYKAQGNA WVDKEIFSDW FHHIFVPSVR EHFRTIGLPE DSKAVLLLDS301SRAHPQEAEL VSSNVFTIFL PASVASLVQP MEQGIRRDFM RNFINPPVPL QGPHARYNMN361DAIFSVACAW NAVPSHVFRR AWRKLWPSVA FAEGSSSEEE LEAECFPVKP HNKSFAHILE421LVKEGSSCPG QLRQRQAASW GVAGREAEGG RPPAATSPAE VVWSSEKTPK ADQDGGGDPG481EGEEVAWEQA AVAFDAVLRF AERQPCFSAQ EVGQLRALRA VFRSQQQETV GLEDVVVTSP541EELAIPKCCL EASTETTRTRPLEQKLISEEDLAANDILDYKDDDDKV
556 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
