LSBio
Human VPS9D1 / C16orf7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human VPS9D1 / C16orf7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all VPS9D1 antibodies →
$220.00each
Catalog Number: LS-G82379-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C16orf7(VPS9D1) (NM_004913) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATP-BL | C16orf7 | VPS9 domain containing 1 | VPS9D1
Origin Species
Human
NCBI GenBank
NM_004913
SwissProt
Q9Y2B5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAAGDGTV KPLQSAMKLA NGAIELDTGN RPREAYTEYL RSIHYISQVL LEEVETTKEA
61GETVPPDTSK MLKLAQQCLE RAQSTAAKLG KTRLKPTMPA AAPIPQPAGR HRRVYSDEGG
121KLSPFLPPEI FQKLQGAESQ SCKKELTPLE EASLQNQKLK AAYEARMARL DPSQAMQKTS
181LTLSLQRQMM ENLVIAKARE ETLQRKMEER RLRLQEAANR RFCSQVALTP EEREQRALYA
241AILEYEQDHD WPKHWKAKLK RNPGDLSLVT SLVSHLLSLP DHPIAQLLRR LQCSVYSALY
301PAVSRAAAPA PGCCPPTPNP GSRRLRPSQS LHCMLSPPEP SAAPRPQDSP PTPPLQPGPV
361GSPSPLGDTA SGLPDKDSSF EDLEQFLGTS ERQGRGRGVQ PEPQLQQLKT AVEEIHNAVD
421RLLSLTLLAF EGLNTAASKD RCLACIEEPF FSPLWPLLLA LYRSVHRARE AALSRSMELY
481RNAPPTAIGI PTKLLPQNPE AKGATGYPYC AAAQELGLLV LESCPQKKLE CIVRTLRIIC
541VCAEDYCPTP EATPQAGPPP IAAAAIGADD LLPILSFVVL RSGLPQLVSE CAALEEFIHE
601GYLIGEEGYC LTSLQSALSY VELLPRGGLA K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

631 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.