LSBio
Human TNF Alpha Protein (Recombinant)

Biological Activity LIF, Human stimulates cell proliferation of R&D TF-1 cells. The ED 50 for this effect is less than 0.2ng/mL.

Human TNF Alpha Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemEscherichia coli (Prokaryotic)
Format:Lyophilized
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$302.00each
Catalog Number: LS-G27304-10

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Overview

Description
Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells. Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | macrophage-derived | monocyte-derived | TNFSF2
Origin Species
Human
NCBI GenBank
NM_000594|NP_000585.2
SwissProt
P01375

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
19.7 kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC
61GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD
121ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

180 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 0.2 EU/µg
Format
Lyophilized
Formulation
Lyophilized from PBS
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 2 weeks or at -20°c for up to 3 months.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.