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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-G259-10 10 µg $215 
LS-G259-50 50 µg $285 
Protein

Human TNF Alpha Protein (Recombinant) - LS-G259

Human TNF Alpha Protein (Recombinant) - LS-G259

Description:
TNF Alpha Protein LS-G259 is Recombinant Human TNF Alpha in E. coli It is biologically active and is low in endotoxin Less than 0.1 ng/µg of protein (less than 1EU/µg)..
Price
Catalog Number
$215 
LS-G259-10
Toll Free North America
866-819-4732

Overview

Description:
TNF Alpha Protein LS-G259 is Recombinant Human TNF Alpha in E. coli It is biologically active and is low in endotoxin Less than 0.1 ng/µg of protein (less than 1EU/µg)..

Specifications

Type
Recombinant Protein
Target
TNF Alpha
Species
Human
Modifications
Unmodified. Also available Animal-free.
Predicted Molecular Weight
17.4 kDa
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is <=0.05 ng/ml, corresponding to a specific activity of >=2 x 10^7 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 3 mM Sodium Phosphate, pH 8.0 + 20mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
Store at 4°C for frequent use; -20°C to -80°C for up to 1 year. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Requested From: United States
Date Requested: 12/10/2019