LSBio
Human TGFB1 / TGF Beta 1 Protein (Recombinant His,C-terminus)

Measured by its binding ability in a functional ELISA. Immobilized Human TGF1-His at 10 µg/mL (100 µl/well) can bind biotinylated human TGFβR2-Fch, The EC50 of biotinylated human TGFβR2-Fch is 50-200 ng/mL.

Human TGFB1 / TGF Beta 1 Protein (Recombinant His,C-terminus)

Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
See all TGFB1 antibodies →
$450.00each
Catalog Number: LS-G48429-50

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Overview

Description
TGFB1 / TGF Beta 1 Protein LS-G48429 is a Recombinant Human TGFB1 / TGF Beta 1 produced in HEK 293 Cells Met1-Ser390 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
CED | DPD1 | Diaphyseal dysplasia 1 | LAP | Latency-associated peptide | TGF Beta | TGFbeta | TGF-beta 1 protein | TGF-beta-1 | TGFB | TGFB1
Origin Species
Human
NCBI GenBank
NM_000660
SwissProt
P01137

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
Hexahistidine Tag
Molecular Weight
The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.
Protein Type
Recombinant
Amino Acid Sequence
1Met1-Ser39 0

11 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

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