SMARCA4 / BRG1 Antibody
Catalog Number: LS-C335201
2 sizes / from $293.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SMARCA4 / BRG1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124855-100
Catalog Number: LS-G124855
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRG1(SMARCA4) (NM_001128849), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATP-dependent helicase SMARCA4 | BAF190 | BRG1-associated factor 190A | Brahma protein-like 1 | BRG1 | BRM/SWI2-related gene 1 | HSNF2b | MRD16 | Protein BRG-1 | SNF2-like 4 | SNF2LB | SNF2-BETA | SNF2B | SWI2 | Nuclear protein GRB1 | SNF2 | SNF2L4 | Transcription activator BRG1 | Homeotic gene regulator | Protein brahma homolog 1 | RTPS2 | SMARCA4 | Sucrose nonfermenting-like 4
- Origin Species
- Human
- NCBI GenBank
- NM_003072
- SwissProt
- P51532
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 188 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTPDPPLGG TPRPGPSPGP GPSPGAMLGP SPGPSPGSAH SMMGPSPGPP SAGHPIPTQG61PGGYPQDNMH QMHKPMESMH EKGMSDDPRY NQMKGMGMRS GGHAGMGPPP SPMDQHSQGY121PSPLGGSEHA SSPVPASGPS SGPQMSSGPG GAPLDGADPQ ALGQQNRGPT PFNQNQLHQL181RAQIMAYKML ARGQPLPDHL QMAVQGKRPM PGMQQQMPTL PPPSVSATGP GPGPGPGPGP241GPGPAPPNYS RPHGMGGPNM PPPGPSGVPP GMPGQPPGGP PKPWPEGPMA NAAAPTSTPQ301KLIPPQPTGR PSPAPPAVPP AASPVMPPQT QSPGQPAQPA PMVPLHQKQS RITPIQKPRG361LDPVEILQER EYRLQARIAH RIQELENLPG SLAGDLRTKA TIELKALRLL NFQRQLRQEV421VVCMRRDTAL ETALNAKAYK RSKRQSLREA RITEKLEKQQ KIEQERKRRQ KHQEYLNSIL481QHAKDFKEYH RSVTGKIQKL TKAVATYHAN TEREQKKENE RIEKERMRRL MAEDEEGYRK541LIDQKKDKRL AYLLQQTDEY VANLTELVRQ HKAAQVAKEK KKKKKKKKAE NAEGQTPAIG601PDGEPLDETS QMSDLPVKVI HVESGKILTG TDAPKAGQLE AWLEMNPGYE VAPRSDSEES661GSEEEEEEEE EEQPQAAQPP TLPVEEKKKI PDPDSDDVSE VDARHIIENA KQDVDDEYGV721SQALARGLQS YYAVAHAVTE RVDKQSALMV NGVLKQYQIK GLEWLVSLYN NNLNGILADE781MGLGKTIQTI ALITYLMEHK RINGPFLIIV PLSTLSNWAY EFDKWAPSVV KVSYKGSPAA841RRAFVPQLRS GKFNVLLTTY EYIIKDKHIL AKIRWKYMIV DEGHRMKNHH CKLTQVLNTH901YVAPRRLLLT GTPLQNKLPE LWALLNFLLP TIFKSCSTFE QWFNAPFAMT GEKVDLNEEE961TILIIRRLHK VLRPFLLRRL KKEVEAQLPE KVEYVIKCDM SALQRVLYRH MQAKGVLLTD1021GSEKDKKGKG GTKTLMNTIM QLRKICNHPY MFQHIEESFS EHLGFTGGIV QGLDLYRASG1081KFELLDRILP KLRATNHKVL LFCQMTSLMT IMEDYFAYRG FKYLRLDGTT KAEDRGMLLK1141TFNEPGSEYF IFLLSTRAGG LGLNLQSADT VIIFDSDWNP HQDLQAQDRA HRIGQQNEVR1201VLRLCTVNSV EEKILAAAKY KLNVDQKVIQ AGMFDQKSSS HERRAFLQAI LEHEEQDESR1261HCSTGSGSAS FAHTAPPPAG VNPDLEEPPL KEEDEVPDDE TVNQMIARHE EEFDLFMRMD1321LDRRREEARN PKRKPRLMEE DELPSWIIKD DAEVERLTCE EEEEKMFGRG SRHRKEVDYS1381DSLTEKQWLK KITGKDIHDT ASSVARGLQF QRGLQFCTRA SKAIEEGTLE EIEEEVRQKK1441SSRKRKRDSD AGSSTPTTST RSRDKDDESK KQKKRGRPPA EKLSPNPPNL TKKMKKIVDA1501VIKYKDSSSG RQLSEVFIQL PSRKELPEYY ELIRKPVDFK KIKERIRNHK YRSLNDLEKD1561VMLLCQNAQT FNLEGSLIYE DSIVLQSVFT SVRQKIEKED DSEGEESEEE EEGEEEGSES1621ESRSVKVKIK LGRKEKAQDR LKGGRRRPSR GSRAKPVVSD DDSEEEQEED RSGSGSEEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1679 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
