LSBio
Human PGK1 / Phosphoglycerate Kinase Protein (Recombinant His)

Human PGK1 / Phosphoglycerate Kinase Protein (Recombinant His)

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Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
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$267.00each
Catalog Number: LS-G577-20

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Overview

Description
Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
MIG10 | PGK1 | Primer recognition protein 2 | PRP 2 | PGKA | Phosphoglycerate kinase 1
Origin Species
Human
NCBI GenBank
NM_000291|NP_000282.1
SwissProt
P00558

Protein

Expression System
Escherichia coli (Prokaryotic)
Fusion Tag
Hexahistidine Tag
Protein Type
Recombinant
Amino Acid Sequence
11-417aa|MG SSHHHHHHSS GLVPRGSHMS LSNKLTLDKL DVKGKRVVMR VDFNVPMKNN
61QITNNQRIKA AVPSIKFCLD NGAKSVVLMS HLGRPDGVPM PDKYSLEPVA VELKSLLGKD
121VLFLKDCVGP EVEKACANPA AGSVILLENL RFHVEEEGKG KDASGNKVKA EPAKIEAFRA
181SLSKLGDVYV NDAFGTAHRA HSSMVGVNLP QKAGGFLMKK ELNYFAKALE SPERPFLAIL
241GGAKVADKIQ LINNMLDKVN EMIIGGGMAF TFLKVLNNME IGTSLFDEEG AKIVKDLMSK
301AEKNGVKITL PVDFVTADKF DENAKTGQAT VASGIPAGWM GLDCGPESSK KYAEAVTRAK
361QIVWNGPVGV FEWEAFARGT KALMDEVVKA TSRGCITIIG GGDTATCCAK WNTEDKVSHV
421STGGGASLEL LEGKVLPGVD ALSNI

445 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Liquid
Formulation
20 mM Tris, pH 8.0, 1 mM DTT, 10% Glycerol
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.

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