Human DHFR Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression System	Human Embryonic Kidney 293T
Format:	Lyophilized

$153.00each

Catalog Number: LS-G75503-20

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Overview

Description: This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DHFR (NM_000791) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category: Proteins & Peptides > Proteins > Lysates
Alternative Names: DHFRP1 | Dihydrofolate reductase | DYR | DHFR
Origin Species: Human
NCBI GenBank: NM_000791
SwissProt: P00374

Protein

Expression System: Human Embryonic Kidney 293T
Fusion Tag: c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight: 21.3 kDa
Protein Type: Lysate

Format

Purity: Unpurified
Conjugate/Label: Unconjugated
Endotoxin Level: Not Tested
Format: Lyophilized
Formulation: Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4