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Human CSF1 / MCSF Protein (Recombinant)

Biological Activity M-CSF, Human stimulates cell proliferation of M-NFS-60 cells. The ED 50 for this effect is typically 1-3ng/mL.

Human CSF1 / MCSF Protein (Recombinant)

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Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
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$302.00each
Catalog Number: LS-G27042-10

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Overview

Description
Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis, is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain, and it is only active in the disulfide-linked dimeric form, which is bonded at Cys63. Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by refolding and chromatographic techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
CSF-1 | MCSF | M-CSF | CSF1 | Lanimostim | CSFM
Origin Species
Human
NCBI GenBank
NM_000757|NP_000748.3
SwissProt
P09603

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
28 kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD
61IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN
121VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

159 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Liquid
Formulation
50 mM Tris-HCl, pH 8.0
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 2 weeks or at -20°c for up to 3 months.

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