LSBio
Human CD274 / B7-H1 / PD-L1 Protein (Recombinant)

Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 µg/ml (100 µl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 µg/ml.

Human CD274 / B7-H1 / PD-L1 Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
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$450.00each
Catalog Number: LS-G47614-100

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Overview

Description
CD274 / B7-H1 / PD-L1 Protein LS-G47614 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239. It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Origin Species
Human
NCBI GenBank
NM_014143
SwissProt
Q9NZQ7

Protein

Expression System
Human Embryonic Kidney 293
Molecular Weight
The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the rh B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
Protein Type
Recombinant
Amino Acid Sequence
1Met1-Thr23 9

11 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

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