BAFF / TNFSF13B Antibody
Catalog Number: LS-C18667-100
100 µg (1 mg/mL) / $552.00
Antibody: Rat Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay, Flow Cytometry
Reactivity: Mouse
Format: Liquid
Human BAFF / TNFSF13B Protein (Recombinant Fc, N-terminus)
ProteinLSBio Guarantee
| Expression System | Other |
| Format: | Lyophilized |
$294.00each
Catalog Number: LS-G137855-10
Catalog Number: LS-G137855
For bulk orders or custom formulations:
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- Description
- TNFSF13B/TNFSF20 belongs to the tumor necrosis factor family. It abundantly is expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3 acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- B-cell-activating factor | BAFF | ApoL related ligand TALL-1 | B-lymphocyte stimulator | CD257 | BLYS | Delta4 BAFF | Delta BAFF | TALL-1 | TALL1 | TNFSF13B | ZTNF4 | THANK | B lymphocyte stimulator | CD257 antigen | TNFSF20
- Origin Species
- Hu
- NCBI GenBank
- NM_006573
- SwissProt
- Q9Y275
Protein
- Expression System
- Other
- Fusion Tag
- hFc
- Protein Type
- Recombinant
- Amino Acid Sequence
1GSGSDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN61WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI121SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP181VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KIEGRAVQGP241EETVTQDCLQ LIADSETPTI QKGSYTFVPW LLSFKRGSAL EEKENKILVK ETGYFFIYGQ301VLYTDKTYAM GHLIQRKKVH VFGDELSLVT LFRCIQNMPE TLPNNSCYSA GIAKLEEGDE361LQLAIPRENA QISLDGDVTF FGALKLL
387 aa
Format
- Purity
- >95%
- Conjugate/Label
- Unconj
- Endotoxin Level
- < 1 EU/µg
- Format
- Lyophilized
- Formulation
- Lyophilized from a sterile solution in PBS, pH 7.4.
- Purity Measurement Method
- SDS-PAGE
Storage & Handling
- Storage Conditions
- Before reconstitution: short term; store at room temperature for up to 3 weeks, long term; store at -20°C. After Reconstitution: short term; store at 4°C to 7°C for up to 7 days, long term; Aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
