LSBio
Human ALY / THOC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ALY / THOC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ALYREF antibodies →
$153.00each
Catalog Number: LS-G80623-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Aly(ALYREF) (NM_005782) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALY | ALYREF | BEF | BZIP enhancing factor | BZIP-enhancing factor BEF | Ally of AML-1 and LEF-1 | Aly/REF export factor | THO complex 4 | THO complex subunit 4 | REF | THOC4 | Tho4
Origin Species
Human
NCBI GenBank
AF047002
SwissProt
Q86V81

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
26.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADKMDMSLD DIIKLNRSQR GGRGGGRGRG RAGSQGGRGG GAQAAARVNR GGGPIRNRPA
61IARGAAGGGG RNRPAPYSRP KQLPDKWQHD LFDSGFGGGA GVETGGKLLV SNLDFGVSDA
121DIQELFAEFG TLKKAAVHYD RSGRSLGTAD VHFERKADAL KAMKQYNGVP LDGRPMNIQL
181VTSQIDAQRR PAQSVNRGGM TRNRGAGGFG GGGGTRRGTR GGARGRGRGA GRNSKQQLSA
241EELDAQLDAY NARMDTS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

257 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.