Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HPTPB / PTPRB Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124177-100
Catalog Number: LS-G124177
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPRB (NM_001109754), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPTPB | PTPB | Ptpbeta | PTPRB | VEPTP | R-PTP-BETA | VE-PTP | HPTP-BETA
- Origin Species
- Human
- NCBI GenBank
- NM_002837
- SwissProt
- P23467
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 249 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAEFYMVIL TCLIFRNSEG FQIVHVQKQQ CLFKNEKVVV GSCNRTIQNQ QWMWTEDEKL61LHVKSALCLA ISNSSRGPSR SAILDRCSQA PRWTCYDQEG FLEVENASLF LQKQGSRVVV121KKARKYLHSW MKIDVNKEGK LVNESLCLQK AGLGAEVSVR STRNTAPPQI LTTFNAVPDG181LVFLIRNTTE AFIRNAAENY SQNSSERQHP NLHMTGITDT SWVLSTTQPF SSTTEETGLA241EPERCNFTLA ESKASSHSVS IQWRILGSPC NFSLIYSSDT LGAALCPTFR IDNTTYGCNL301QDLQAGTIYN FRIISLDEER TVVLQTDPLP PARFGVSKEK TTSTSLHVWW TPSSGKVTSY361EVQLFDENNQ KIQGVQIQES TSWNEYTFFN LTAGSKYNIA ITAVSGGKRS FSVYTNGSTV421PSPVKDIGIS TKANSLLISW SHGSGNVERY RLMLMDKGIL VHGGVVDKHA TSYAFHGLTP481GYLYNLTVMT EAAGLQNYRW KLVRTAPMEV SNLKVTNDGS LTSLKVKWQR PPGNVDSYNI541TLSHKGTIKE SRVLAPWITE THFKELVPGR LYQVTVSCVS GELSAQKMAV GRTFPDKVAN601LEANNNGRMR SLVVSWSPPA GDWEQYRILL FNDSVVLLNI TVGKEETQYV MDDTGLVPGR661QYEVEVIVES GNLKNSERCQ GRTVPLAVLQ LRVKHANETS LSIMWQTPVA EWEKYIISLA721DRDLLLIHKS LSKDAKEFTF TDLVPGRKYM ATVTSISGDL KNSSSVKGRT VPAQVTDLHV781ANQGMTSSLF TNWTQAQGDV EFYQVLLIHE NVVIKNESIS SETSRYSFHS LKSGSLYSVV841VTTVSGGISS RQVVVEGRTV PSSVSGVTVN NSGRNDYLSV SWLLAPGDVD NYEVTLSHDG901KVVQSLVIAK SVRECSFSSL TPGRLYTVTI TTRSGKYENH SFSQERTVPD KVQGVSVSNS961ARSDYLRVSW VHATGDFDHY EVTIKNKNNF IQTKSIPKSE NECVFVQLVP GRLYSVTVTT1021KSGQYEANEQ GNGRTIPEPV KDLTLRNRST EDLHVTWSGA NGDVDQYEIQ LLFNDMKVFP1081PFHLVNTATE YRFTSLTPGR QYKILVLTIS GDVQQSAFIE GFTVPSAVKN IHISPNGATD1141SLTVNWTPGG GDVDSYTVSA FRHSQKVDSQ TIPKHVFEHT FHRLEAGEQY QIMIASVSGS1201LKNQINVVGR TVPASVQGVI ADNAYSSYSL IVSWQKAAGV AERYDILLLT ENGILLRNTS1261EPATTKQHKF EDLTPGKKYK IQILTVSGGL FSKEAQTEGR TVPAAVTDLR ITENSTRHLS1321FRWTASEGEL SWYNIFLYNP DGNLQERAQV DPLVQSFSFQ NLLQGRMYKM VIVTHSGELS1381NESFIFGRTV PASVSHLRGS NRNTTDSLWF NWSPASGDFD FYELILYNPN GTKKENWKDK1441DLTEWRFQGL VPGRKYVLWV VTHSGDLSNK VTAESRTAPS PPSLMSFADI ANTSLAITWK1501GPPDWTDYND FELQWLPRDA LTVFNPYNNR KSEGRIVYGL RPGRSYQFNV KTVSGDSWKT1561YSKPIFGSVR TKPDKIQNLH CRPQNSTAIA CSWIPPDSDF DGYSIECRKM DTQEVEFSRK1621LEKEKSLLNI MMLVPHKRYL VSIKVQSAGM TSEVVEDSTI TMIDRPPPPP PHIRVNEKDV1681LISKSSINFT VNCSWFSDTN GAVKYFTVVV READGSDELK PEQQHPLPSY LEYRHNASIR1741VYQTNYFASK CAENPNSNSK SFNIKLGAEM ESLGGKCDPT QQKFCDGPLK PHTAYRISIR1801AFTQLFDEDL KEFTKPLYSD TFFSLPITTE SEPLFGAIEG VSAGLFLIGM LVAVVALLIC1861RQKVSHGRER PSARLSIRRD RPLSVHLNLG QKGNRKTSCP IKINQFEGHF MKLQADSNYL1921LSKEYEELKD VGRNQSCDIA LLPENRGKNR YNNILPYDAT RVKLSNVDDD PCSDYINASY1981IPGNNFRREY IVTQGPLPGT KDDFWKMVWE QNVHNIVMVT QCVEKGRVKC DHYWPADQDS2041LYYGDLILQM LSESVLPEWT IREFKICGEE QLDAHRLIRH FHYTVWPDHG VPETTQSLIQ2101FVRTVRDYIN RSPGAGPTVV HCSAGVGRTG TFIALDRILQ QLDSKDSVDI YGAVHDLRLH2161RVHMVQTECQ YVYLHQCVRD VLRARKLRSE QENPLFPIYE NVNPEYHRDP VYSRHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2215 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Lysate samples are stable for 12 months when stored at -80°C.