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HLA-B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HLA-B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100575-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HLAB(HLA) (NM_005514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HLAB | MHC Class I HLA heavy chain | Leukocyte antigen class I-B | SPDA1
Origin Species
Hu
NCBI GenBank
NM_005514
SwissProt
P01889

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
40.33 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLVMAPRTVL LLLSAALALT ETWAGSHSMR YFDTAMSRPG RGEPRFISVG YVDDTQFVRF
61DSDAASPREE PRAPWIEQEG PEYWDRNTQI FKTNTQTDRE SLRNLRGYYN QSEAGSHTLQ
121SMYGCDVGPD GRLLRGHNQY AYDGKDYIAL NEDLRSWTAA DTAAQITQRK WEAARVAEQD
181RAYLEGTCVE WLRRYLENGK DTLERADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT
241WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP
301SSQSTVPIVG IVAGLAVLAV VVIGAVVAAV MCRRKSSGGK GGSYSQAACS DSAQGSDVSL
361TA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

362 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.