RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110745-100
Catalog Number: LS-G110745
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRIP1 (NM_021150), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRIP | GRIP-1 | GRIP1
- Origin Species
- Human
- NCBI GenBank
- AJ133439
- SwissProt
- Q9Y3R0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIAVSFKCRC QILRRLTKDE SPYTKSASQT KPPDGALAVR RQSIPEEFKG STVVELMKKE61GTTLGLTVSG GIDKDGKPRV SNLRQGGIAA RSDQLDVGDY IKAVNGINLA KFRHDEIISL121LKNVGERVVL EVEYELPPVS VQGSSVIFRT VEVTLHKEGN TFGFVIRGGA HDDRNKSRPV181VITCVRPGGP ADREGTIKPG DRLLSVDGIR LLGTTHAEAM SILKQCGQEA ALLIEYDVSV241MDSVATASGP LLVEVAKTPG ASLGVALTTS MCCNKQVIVI DKIKSASIAD RCGALHVGDH301ILSIDGTSME YCTLAEATQF LANTTDQVKL EILPHHQTRL ALKGPDHAAL VSSSFSPTSM361SAYSLSSLNM GTLPRSLYST SPRGTMMRRR LKKKDFKSSL SLASSTVGLA GQVVHTETTE421VVLTADPVTG FGIQLQGSVF ATETLSSPPL ISYIEADSPA ERCGVLQIGD RVMAINGIPT481EDSTFEEASQ LLRDSSITSK VTLEIEFDVA ESVIPSSGTF HVKLPKKHNV ELGITISSPS541SRKPGDPLVI SDIKKGSVAH RTGTLELGDK LLAIDNIRLD NCSMEDAVQI LQQCEDLVKL601KIRKDEDNSD EQESSGAIIY TVELKRYGGP LGITISGTEE PFDPIIISSL TKGGLAERTG661AIHIGDRILA INSSSLKGKP LSEAIHLLQM AGETVTLKIK KQTDAQSASS PKKFPISSHL721SDLGDVEEDS SPAQKPGKLS DMYPSTVPSV DSAVDSWDGS AIDTSYGTEG TSFQASGYNF781NTYDWRSPKQ RGSLSPVTKP RSQTYPDVGL SYEDWDRSTA SGFAGAADSA ETEQEENFWS841QALEDLETCG QSGILRELEE KADRRVSLRN MTLLATIMSG STMSLNHEAP TPRSQLGRQA901SFQERSSSRP HYSQTTRSNT LPSDVGRKSV TLRKMKQEIK EIMSPTPVEL HKVTLYKDSD961MEDFGFSVAD GLLEKGVYVK NIRPAGPGDL GGLKPYDRLL QVNHVRTRDF DCCLVVPLIA1021ESGNKLDLVI SRNPLASQKS IDQQSLPGDW SEQNSAFFQQ PSHGGNLETR EPTNTLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1076 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
