Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIN2C / NMDAR2C / NR2C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122971-100
Catalog Number: LS-G122971
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NMDAR2C(GRIN2C) (NM_000835), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRIN2C | GluN2C | NMDA receptor 2C | NMDAR2C | NR2C | GluR epsilon 3
- Origin Species
- Human
- NCBI GenBank
- NM_000835
- SwissProt
- Q14957
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.21 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGALGPALL LTSLFGAWAG LGPGQGEQGM TVAVVFSSSG PPQAQFRARL TPQSFLDLPL61EIQPLTVGVN TTNPSSLLTQ ICGLLGAAHV HGIVFEDNVD TEAVAQILDF ISSQTHVPIL121SISGGSAVVL TPKEPGSAFL QLGVSLEQQL QVLFKVLEEY DWSAFAVITS LHPGHALFLE181GVRAVADASH VSWRLLDVVT LELGPGGPRA RTQRLLRQLD APVFVAYCSR EEAEVLFAEA241AQAGLVGPGH VWLVPNLALG STDAPPATFP VGLISVVTES WRLSLRQKVR DGVAILALGA301HSYWRQHGTL PAPAGDCRVH PGPVSPAREA FYRHLLNVTW EGRDFSFSPG GYLVQPTMVV361IALNRHRLWE MVGRWEHGVL YMKYPVWPRY SASLQPVVDS RHLTVATLEE RPFVIVESPD421PGTGGCVPNT VPCRRQSNHT FSSGDVAPYT KLCCKGFCID ILKKLARVVK FSYDLYLVTN481GKHGKRVRGV WNGMIGEVYY KRADMAIGSL TINEERSEIV DFSVPFVETG ISVMVARSNG541TVSPSAFLEP YSPAVWVMMF VMCLTVVAIT VFMFEYFSPV SYNQNLTRGK KSGGPAFTIG601KSVWLLWALV FNNSVPIENP RGTTSKIMVL VWAFFAVIFL ASYTANLAAF MIQEQYIDTV661SGLSDKKFQR PQDQYPPFRF GTVPNGSTER NIRSNYRDMH THMVKFNQRS VEDALTSLKM721GKLDAFIYDA AVLNYMAGKD EGCKLVTIGS GKVFATTGYG IAMQKDSHWK RAIDLALLQF781LGDGETQKLE TVWLSGICQN EKNEVMSSKL DIDNMAGVFY MLLVAMGLAL LVFAWEHLVY841WKLRHSVPNS SQLDFLLAFS RGIYSCFSGV QSLASPPRQA SPDLTASSAQ ASVLKMLQAA901RDMVTTAGVS SSLDRATRTI ENWGGGRRAP PPSPCPTPRS GPSPCLPTPD PPPEPSPTGW961GPPDGGRAAL VRRAPQPPGR PPTPGPPLSD VSRVSRRPAW EARWPVRTGH CGRHLSASER1021PLSPARCHYS SFPRADRSGR PFLPLFPELE DLPLLGPEQL ARREALLHAA WARGSRPRHA1081SLPSSVAEAF ARPSSLPAGC TGPACARPDG HSACRRLAQA QSMCLPIYRE ACQEGEQAGA1141PAWQHRQHVC LHAHAHLPFC WGAVCPHLPP CASHGSWLSG AWGPLGHRGR TLGLGTGYRD1201SGGLDEISRV ARGTQGFPGP CTWRRISSLE SEVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1233 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.