RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR142 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104381-100
Catalog Number: LS-G104381
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR142 (NM_181790), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 142 | GPR142 | PGR2
- Origin Species
- Human
- NCBI GenBank
- NM_181790
- SwissProt
- Q7Z601
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSIMMLPMEQ KIQWVPTSLQ DITAVLGTEA YTEEDKSMVS HAQKSQHSCL SHSRWLRSPQ61VTGGSWDLRI RPSKDSSSFR QAQCLRKDPG ANNHLESQGV RGTAGDADRE LRGPSEKATA121GQPRVTLLPT PHVSGLSQEF ESHWPEIAER SPCVAGVIPV IYYSVLLGLG LPVSLLTAVA181LARLATRTRR PSYYYLLALT ASDIIIQVVI VFAGFLLQGA VLARQVPQAV VRTANILEFA241ANHASVWIAI LLTVDRYTAL CHPLHHRAAS SPGRTRRAIA AVLSAALLTG IPFYWWLDMW301RDTDSPRTLD EVLKWAHCLT VYFIPCGVFL VTNSAIIHRL RRRGRSGLQP RVGKSTAILL361GITTLFTLLW APRVFVMLYH MYVAPVHRDW RVHLALDVAN MVAMLHTAAN FGLYCFVSKT421FRATVRQVIH DAYLPCTLAS QPEGMAAKPV MEPPGLPTGA EVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
462 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
