LSBio
GPI Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPI Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPI antibodies →
$504.00each
Catalog Number: LS-G130027-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glucose 6 phosphate isomerase(GPI) (NM_001184722), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AMF | Autocrine motility factor | Glucose phosphate isomerase | Hexose monophosphate isomerase | Hexosephosphate isomerase | GPI | Neuroleukin | Oxoisomerase | Phosphoglucose isomerase | Phosphohexomutase | Phosphohexose isomerase | SA36 | PGI | SA-36 | Glucose-6-phosphate isomerase | GNPI | PHI | Phosphosaccharomutase | Sperm antigen 36 | Sperm antigen-36
Origin Species
Human
NCBI GenBank
NM_000175
SwissProt
P06744

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVALCSLQHL GSSDPRALPT LPTATSGQRP AKRRRKSPAM AALTRDPQFQ KLQQWYREHR
61SELNLRRLFD ANKDRFNHFS LTLNTNHGHI LVDYSKNLVT EDVMRMLVDL AKSRGVEAAR
121ERMFNGEKIN YTEGRAVLHV ALRNRSNTPI LVDGKDVMPE VNKVLDKMKS FCQGPLMVTE
181ALKPYSSGGP RVWYVSNIDG THIAKTLAQL NPESSLFIIA SKTFTTQETI TNAETAKEWF
241LQAAKDPSAV AKHFVALSTN TTKVKEFGID PQNMFEFWDW VGGRYSLWSA IGLSIALHVG
301FDNFEQLLSG AHWMDQHFRT TPLEKNAPVL LALLGIWYIN CFGCETHAML PYDQYLHRFA
361AYFQQGDMES NGKYITKSGT RVDHQTGPIV WGEPGTNGQH AFYQLIHQGT KMIPCDFLIP
421VQTQHPIRKG LHHKILLANF LAQTEALMRG KSTEEARKEL QAAGKSPEDL ERLLPHKVFE
481GNRPTNSIVF TKLTPFMLGA LVAMYEHKIF VQGIIWDINS FDQWGVELGK QLAKKIEPEL
541DGSAQVTSHD ASTNGLINFI KQQREARVQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.