RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GNAL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104058-100
Catalog Number: LS-G104058
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GNAL (NM_182978), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GNAL
- Origin Species
- Human
- NCBI GenBank
- NM_002071
- SwissProt
- P38405
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLCYSLRPL LFGGPGDDPC AASEPPVEDA QPAPAPALAP VRAAARDTAR TLLPRGGEGS61PACARPKADK PKEKRQRTEQ LSAEEREAAK EREAVKEARK VSRGIDRMLR DQKRDLQQTH121RLLLLGAGES GKSTIVKQMR ILHVNGFNPE EKKQKILDIR KNVKDAIVTI VSAMSTIIPP181VPLANPENQF RSDYIKSIAP ITDFEYSQEF FDHVKKLWDD EGVKACFERS NEYQLIDCAQ241YFLERIDSVS LVDYTPTDQD LLRCRVLTSG IFETRFQVDK VNFHMFDVGG QRDERKKWIQ301CFNDVTAIIY VAACSSYNMV IREDNNTNRL RESLDLFESI WNNRWLRTIS IILFLNKQDM361LAEKVLAGKS KIEDYFPEYA NYTVPEDATP DAGEDPKVTR AKFFIRDLFL RISTATGDGK421HYCYPHFTCA VDTENIRRVF NDCRDIIQRM HLKQYELLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
458 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
