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FSTL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FSTL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113487-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FSTL4 (NM_015082), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Follistatin-like 4 | Follistatin-related protein 4 | FSTL4 | Follistatin-like protein 4 | KIAA1061
Origin Species
Human
NCBI GenBank
AB028984
SwissProt
Q6MZW2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPGGFWLHL TLLGASLPAA LGWMDPGTSR GPDVGVGESQ AEEPRSFEVT RREGLSSHNE
61LLASCGKKFC SRGSRCVLSR KTGEPECQCL EACRPSYVPV CGSDGRFYEN HCKLHRAACL
121LGKRITVIHS KDCFLKGDTC TMAGYARLKN VLLALQTRLQ PLQEGDSRQD PASQKRLLVE
181SLFRDLDADG NGHLSSSELA QHVLKKQDLD EDLLGCSPGD LLRFDDYNSD SSLTLREFYM
241AFQVVQLSLA PEDRVSVTTV TVGLSTVLTC AVHGDLRPPI IWKRNGLTLN FLDLEDINDF
301GEDDSLYITK VTTIHMGNYT CHASGHEQLF QTHVLQVNVP PVIRVYPESQ AQEPGVAASL
361RCHAEGIPMP RITWLKNGVD VSTQMSKQLS LLANGSELHI SSVRYEDTGA YTCIAKNEVG
421VDEDISSLFI EDSARKTLAN ILWREEGLSV GNMFYVFSDD GIIVIHPVDC EIQRHLKPTE
481KIFMSYEEIC PQREKNATQP CQWVSAVNVR NRYIYVAQPA LSRVLVVDIQ AQKVLQSIGV
541DPLPAKLSYD KSHDQVWVLS WGDVHKSRPS LQVITEASTG QSQHLIRTPF AGVDDFFIPP
601TNLIINHIRF GFIFNKSDPA VHKVDLETMM PLKTIGLHHH GCVPQAMAHT HLGGYFFIQC
661RQDSPASAAR QLLVDSVTDS VLGPNGDVTG TPHTSPDGRF IVSAAADSPW LHVQEITVRG
721EIQTLYDLQI NSGISDLAFQ RSFTESNQYN IYAALHTEPD LLFLELSTGK VGMLKNLKEP
781PAGPAQPWGG THRIMRDSGL FGQYLLTPAR ESLFLINGRQ NTLRCEVSGI KGGTTVVWVG
841EV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

842 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.