Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FOLH1 / PSMA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130954-100
Catalog Number: LS-G130954
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSMA(FOLH1) (NM_001193473), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Folate hydrolase 1 | FOLH1 | GCPII | Glutamate carboxypeptidase II | Folate hydrolase | GCP2 | NAALAD1 | NAALAdase | NAALADase I | PSM | FGCP | FOLH | Glutamate carboxylase II | Glutamate carboxypeptidase 2 | MGCP | PSMA
- Origin Species
- Human
- NCBI GenBank
- NM_004476
- SwissProt
- Q04609
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGSAPPDSS WRGSLKVPYN VGPGFTGNFS TQKVKMHIHS TNEVTRIYNV IGTLRGAVEP61DRYVILGGHR DSWVFGGIDP QSGAAVVHEI VRSFGTLKKE GWRPRRTILF ASWDAEEFGL121LGSTEWAEEN SRLLQERGVA YINADSSIEG NYTLRVDCTP LMYSLVHNLT KELKSPDEGF181EGKSLYESWT KKSPSPEFSG MPRISKLGSG NDFEVFFQRL GIASGRARYT KNWETNKFSG241YPLYHSVYET YELVEKFYDP MFKYHLTVAQ VRGGMVFELA NSIVLPFDCR DYAVVLRKYA301DKIYSISMKH PQEMKTYSVS FDSLFSAVKN FTEIASKFSE RLQDFDKSNP IVLRMMNDQL361MFLERAFIDP LGLPDRPFYR HVIYAPSSHN KYAGESFPGI YDALFDIESK VDPSKAWGEV421KRQIYVAAFT VQAAAETLSE VATRTRPLEQKLISEEDLAANDILDYKDDDDKV
442 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.