RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EYA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127465-100
Catalog Number: LS-G127465
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EYA1 (NM_172060), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BOP | BOS1 | EYA1 | Eyes absent homolog 1 | BOR
- Origin Species
- Human
- NCBI GenBank
- NM_000503
- SwissProt
- Q99502
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLFPQVAVK TEPMSSSETA STTADGSLNN FSGSAIGSSS FSPRPTHQFS PPQIYPSNRP61YPHILPTPSS QTMAAYGQTQ FTTGMQQATA YATYPQPGQP YGISSYGIKT EGGLSQSQSP121GQTGFLSYGT SFSTPQPGQA PYSYQMQGSS FTTSSGIYTG NNSLTNSSGF NSSQQDYPSY181PSFGQGQYAQ YYNSSPYPAH YMTSSNTSPT TPSTNATYQL QEPPSGITSQ AVTDPTAEYS241TIHSPSTPIK DSDSDRLRRG SDGKSRGRGR RNNNPSPPPD SDLERVFIWD LDETIIVFHS301LLTGSYANRY GRDPPTSVSL GLRMEEMIFN LADTHLFFND LEECDQVHID DVSSDDNGQD361LSTYNFGTDG FPAAATSANL CLATGVRGGV DWMRKLAFRY RRVKEIYNTY KNNVGGLLGP421AKREAWLQLR AEIEALTDSW LTLALKALSL IHSRTNCVNI LVTTTQLIPA LAKVLLYGLG481IVFPIENIYS ATKIGKESCF ERIIQRFGRK VVYVVIGDGV EEEQGAKKAR DALLEDLQPL541GPHGPAPRLG TGVPVTALGT LTAHSCSVTR DRSSRPQSRI SAGLQNLAIR RNTLNWWMQQ601MTRTRPLEQKLISEEDLAANDILDYKDDDDKV
601 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
