RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EYA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105549-100
Catalog Number: LS-G105549
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EYA1 (NM_172059), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BOP | BOS1 | EYA1 | Eyes absent homolog 1 | BOR
- Origin Species
- Human
- NCBI GenBank
- NM_000503
- SwissProt
- Q99502
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEMQDLTSPH SRLSGSSESP SGPKLGNSHI NSNSMTPNGT EVKTEPMSSS ETASTTADGS61LNNFSGSAIG SSSFSPRPTH QFSPPQIYPS NRPYPHILPT PSSQTMAAYG QTQFTTGMQQ121ATAYATYPQP GQPYGISSYG IKTEGGLSQS QSPGQTGFLS YGTSFSTPQP GQAPYSYQMQ181GSSFTTSSGI YTGNNSLTNS SGFNSSQQDY PSYPSFGQGQ YAQYYNSSPY PAHYMTSSNT241SPTTPSTNAT YQLQEPPSGI TSQAVTDPTA EYSTIHSPST PIKDSDSDRL RRGSDGKSRG301RGRRNNNPSP PPDSDLERVF IWDLDETIIV FHSLLTGSYA NRYGRECDQV HIDDVSSDDN361GQDLSTYNFG TDGFPAAATS ANLCLATGVR GGVDWMRKLA FRYRRVKEIY NTYKNNVGGL421LGPAKREAWL QLRAEIEALT DSWLTLALKA LSLIHSRTNC VNILVTTTQL IPALAKVLLY481GLGIVFPIEN IYSATKIGKE SCFERIIQRF GRKVVYVVIG DGVEEEQGAK KHAMPFWRIS541SHSDLMALHH ALELEYLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
557 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
