Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERCC2 / XPD Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125142-100
Catalog Number: LS-G125142
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human XPD(ERCC2) (NM_001130867), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COFS2 | EM9 | ERCC2 | XPD | TFIIH p80 | BTF2 p80 | TTD | XPDC | CXPD | TFIIH 80 kDa subunit
- Origin Species
- Human
- NCBI GenBank
- NM_000400
- SwissProt
- P18074
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRELKRTLDA KGHGVLEMPS GTGKTVSLLA LIMAYQRAYP LEVTKLIYCS RTVPEIEKVI61EELRKLLNFY EKQEGEKLPF LGLALSSRKN LCIHPEVTPL RFGKDVDGKC HSLTASYVRA121QYQHDTSLPH CRFYEEFDAH GREVPLPAGI YNLDDLKALG RRQGWCPYFL ARYSILHANV181VVYSYHYLLD PKIADLVSKE LARKAVVVFD EAHNIDNVCI DSMSVNLTRR TLDRCQGNLE241TLQKTVLRIK ETDEQRLRDE YRRLVEGLRE ASAARETDAH LANPVLPDEV LQEAVPGSIR301TAEHFLGFLR RLLEYVKWRL RVQHVVQESP PAFLSGLAQR VCIQRKPLRF CAERLRSLLH361TLEITDLADF SPLTLLANFA TLVSTYAKGQ AQHCGSSRNQ KRSHPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
405 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.