RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERAL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100623-100
Catalog Number: LS-G100623
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTP binding protein era homolog(ERAL1) (NM_005702), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Conserved ERA-like GTPase | CEGA | Era | Era G-protein-like 1 (E. coli) | ERAL1 | GTPase Era | mitochondrial | H-ERA | ERA-W | HERA | HERA-B | ERA-like protein 1 | ERAL1A | HERA-A
- Origin Species
- Human
- NCBI GenBank
- AA758213
- SwissProt
- O75616
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPSWRGAR LVQSVLRVWQ VGPHVARERV IPFSSLLGFQ RRCVSCVAGS AFSGPRLASA61SRSNGQGSAL DHFLGFSQPD SSVTPCVPAV SMNRDEQDVL LVHHPDMPEN SRVLRVVLLG121APNAGKSTLS NQLLGRKVFP VSRKVHTTRC QALGVITEKE TQVILLDTPG IISPGKQKRH181HLELSLLEDP WKSMESADLV VVLVDVSDKW TRNQLSPQLL RCLTKYSQIP SVLVMNKVDC241LKQKSVLLEL TAALTEGVVN GKKLKMRQAF HSHPGTHCPS PAVKDPNTQS VGNPQRIGWP301HFKEIFMLSA LSQEDVKTLK QYLLTQAQPG PWEYHSAVLT SQTPEEICAN IIREKLLEHL361PQEVPYNVQQ KTAVWEEGPG GELVIQQKLL VPKESYVKLL IGPKGHVISQ IAQEAGHDLM421DIFLCDVDIR LSVKLLKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
437 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
