Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125494-100
Catalog Number: LS-G125494
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PKR(EIF2AK2) (NM_001135652), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EIF2AK2 | p1/eIF-2A protein kinase | p68 kinase | PRKR | EIF-2A protein kinase 2 | p68 | Protein kinase RNA-activated | Tik
- Origin Species
- Human
- NCBI GenBank
- NM_002759
- SwissProt
- P19525
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGDLSAGFF MEELNTYRQK QGVVLKYQEL PNSGPPHDRR FTFQVIIDGR EFPEGEGRSK61KEAKNAAAKL AVEILNKEKK AVSPLLLTTT NSSEGLSMGN YIGLINRIAQ KKRLTVNYEQ121CASGVHGPEG FHYKCKMGQK EYSIGTGSTK QEAKQLAAKL AYLQILSEET SVKSDYLSSG181SFATTCESQS NSLVTSTLAS ESSSEGDFSA DTSEINSNSD SLNSSSLLMN GLRNNQRKAK241RSLAPRFDLP DMKETKYTVD KRKAEREVKA LAKLDHVNIV HYNGCWDGFD YDPETSDDSL301ESSDYDPENS KNSSRSKTKC LFIQMEFCDK GTLEQWIEKR RGEKLDKVLA LELFEQITKG361VDYIHSKKLI HRDLKPSNIF LVDTKQVKIG DFGLVTSLKN DGKRTRSKGT LRYMSPEQIS421SQDYGKEVDL YALGLILAEL LHVCDTAFET SKFFTDLRDG IISDIFDKKE KTLLQKLLSK481KPEDRPNTSE ILRTLTVWKK SPEKNERHTCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
510 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.