RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EHD4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102265-100
Catalog Number: LS-G102265
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EHD4 (NM_139265), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EH domain-containing protein 4 | EH-domain containing 4 | EH domain containing 4 | EHD4 | HCA10 | HCA11 | PAST4 | Ortholog of rat pincher | PAST homolog 4
- Origin Species
- Human
- NCBI GenBank
- NM_139265
- SwissProt
- Q9H223
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFSWMGRQAG GRERAGGADA VQTVTGGLRS LYLRKVLPLE EAYRFHEFHS PALEDADFEN61KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF IAVMYGETEG STPGNALVVD121PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK SISVIDSPGI LSGEKQRISR GYDFCQVLQW181FAERVDRIIL LFDAHKLDIS DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM241WSLGKVINTP EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL301IKRARLAKVH AYIISYLKKE MPSVFGKENK KRELISRLPE IYIQLQREYQ ISAGDFPEVK361AMQEQLENYD FTKFHSLKPK LIEAVDNMLS NKISPLMNLI SQEETSTPTQ LVQGGAFDGT421TEGPFNQGYG EGAKEGADEE EWVVAKDKPV YDELFYTLSP INGKISGVNA KKEMVTSKLP481NSVLGKIWKL ADCDCDGMLD EEEFALAKHL IKIKLDGYEL PSSLPPHLVP PSHRKSLPKA541DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
541 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
