RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EHD3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101236-100
Catalog Number: LS-G101236
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EHD3 (NM_014600), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EH domain containing 3 | EH domain-containing protein 3 | EH-domain containing 3 | PAST homolog 3 | EHD3 | PAST3
- Origin Species
- Human
- NCBI GenBank
- NM_014600
- SwissProt
- Q9NZN3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFSWLGTDDR RRKDPEVFQT VSEGLKKLYK SKLLPLEEHY RFHEFHSPAL EDADFDNKPM61VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV MQGDMEGIIP GNALVVDPKK121PFRKLNAFGN AFLNRFVCAQ LPNPVLESIS VIDTPGILSG EKQRISRGYD FAAVLEWFAE181RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL241GKIVNTPEVI RVYIGSFWSH PLLIPDNRKL FEAEEQDLFR DIQSLPRNAA LRKLNDLIKR301ARLAKVHAYI ISSLKKEMPS VFGKDNKKKE LVNNLAEIYG RIEREHQISP GDFPNLKRMQ361DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE ESQRPIQMVK GGAFEGTLHG421PFGHGYGEGA GEGIDDAEWV VARDKPMYDE IFYTLSPVDG KITGANAKKE MVRSKLPNSV481LGKIWKLADI DKDGMLDDDE FALANHLIKV KLEGHELPNE LPAHLLPPSK RKVAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
535 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
