RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EHBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113348-100
Catalog Number: LS-G113348
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EHBP1 (NM_015252), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EH domain binding protein 1 | EH domain-binding protein 1 | EHBP1 | HPC12 | NPF calponin-like protein | KIAA0903 | NACSIN
- Origin Species
- Human
- NCBI GenBank
- AK000828
- SwissProt
- Q8NDI1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 139.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASVWKRLQR VGKHASKFQF VASYQELMVE CTKKWQPDKL VVVWTRRSRR KSSKAHSWQP61GIKNPYRGVV VWPVPENIEI TVTLFKDPHA EEFEDKEWTF VIENESPSGR RKALATSSIN121MKQYASPMPT QTDVKLKFKP LSKKVVSAAL QFSLSCIFLR EGKATDEDMQ SLASLMSMKQ181ADIGNLDDFE EDNEDDDENR VNQEEKAAKI TEIVNQLNAL SSLDEDQDDC IKQANMRSAK241SASSSEELIN KLNFLDEAEK DLATVNSNPF DDPDAAELNP FGDPDSEEPI TETASPRKTE301DSFYNNSYNP FKEVQTPQYL NPFDEPEAFV TIKDSPPQST KRKNIRPVDM SKYLYADSSK361TEEEELDESN PFYEPKSTPP PNNLVNPVQE LETERRVKRK APAPPVLSPK TGVLNENTVS421AGKDLSTSPK PSPIPSPVLG RKPNASQSLL VWCKEVTKNY RGVKITNFTT SWRNGLSFCA481ILHHFRPDLI DYKSLNPQDI KENNKKAYDG FASIGISRLL EPSDMVLLAI PDKLTVMTYL541YQIRAHFSGQ ELNVVQIEEN SSKSTYKVGN YETDTNSSVD QEKFYAELSD LKREPELQQP601ISGAVDFLSQ DDSVFVNDSG VGESESEHQT PDDHLSPSTA SPYCRRTKSD TEPQKSQQSS661GRTSGSDDPG ICSNTDSTQA QVLLGKKRLL KAETLELSDL YVSDKKKDMS PPFICEETDE721QKLQTLDIGS NLEKEKLENS RSLECRSDPE SPIKKTSLSP TSKLGYSYSR DLDLAKKKHA781SLRQTESDPD ADRTTLNHAD HSSKIVQHRL LSRQEELKER ARVLLEQARR DAALKAGNKH841NTNTATPFCN RQLSDQQDEE RRRQLRERAR QLIAEARSGV KMSELPSYGE MAAEKLKERS901KASGDENDNI EIDTNEEIPE GFVVGGGDEL TNLENDLDTP EQNSKLVDLK LKKLLEVQPQ961VANSPSSAAQ KAVTESSEQD MKSGTEDLRT ERLQKTTERF RNPVVFSKDS TVRKTQLQSF1021SQYIENRPEM KRQRSIQEDT KKGNEEKAAI TETQRKPSED EVLNKGFKDT SQYVVGELAA1081LENEQKQIDT RAALVEKRLR YLMDTGRNTE EEEAMMQEWF MLVNKKNALI RRMNQLSLLE1141KEHDLERRYE LLNRELRAML AIEDWQKTEA QKRREQLLLD ELVALVNKRD ALVRDLDAQE1201KQAEEEDEHL ERTLEQNKGK MAKKEEKCVL QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1231 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
