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ECE-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ECE-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124274-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ECE1 (NM_001113349), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ECE-1 | ECE | ET-converting enzyme-1 | Endothelin converting enzyme 1 | Endothelin-converting enzyme | ET-1 converting enzyme-1 | ECE1 | Endothelin-converting enzyme 1 | HECE-1
Origin Species
Human
NCBI GenBank
NM_001397
SwissProt
P42892

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MEALRESVLH LALQMSTYKR ATLDEEDLVD SLSEGDAYPN GLQVNFHSPR SGQRCWAART
61QVEKRLVVLV VLLAAGLVAC LAALGIQYQT RSPSVCLSEA CVSVTSSILS SMDPTVDPCH
121DFFSYACGGW IKANPVPDGH SRWGTFSNLW EHNQAIIKHL LENSTASVSE AERKAQVYYR
181ACMNETRIEE LRAKPLMELI ERLGGWNITG PWAKDNFQDT LQVVTAHYRT SPFFSVYVSA
241DSKNSNSNVI QVDQSGLGLP SRDYYLNKTE NEKVLTGYLN YMVQLGKLLG GGDEEAIRPQ
301MQQILDFETA LANITIPQEK RRDEELIYHK VTAAELQTLA PAINWLPFLN TIFYPVEINE
361SEPIVVYDKE YLEQISTLIN TTDRCLLNNY MIWNLVRKTS SFLDQRFQDA DEKFMEVMYG
421TKKTCLPRWK FCVSDTENNL GFALGPMFVK ATFAEDSKSI ATEIILEIKK AFEESLSTLK
481WMDEETRKSA KEKADAIYNM IGYPNFIMDP KELDKVFNDY TAVPDLYFEN AMRFFNFSWR
541VTADQLRKAP NRDQWSMTPP MVNAYYSPTK NEIVFPAGIL QAPFYTRSSP KALNFGGIGV
601VVGHELTHAF DDQGREYDKD GNLRPWWKNS SVEAFKRQTE CMVEQYSNYS VNGEPVNGRH
661TLGENIADNG GLKAAYRAYQ NWVKKNGAEH SLPTLGLTNN QLFFLGFAQV WCSVRTPESS
721HEGLITDPHS PSRFRVIGSL SNSKEFSEHF RCPPGSPMNP PHKCEVW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

767 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.